Coding

Part:BBa_K2057002:Design

Designed by: José Eleazar Barboza Corona   Group: iGEM16_Guanajuato_Mx   (2016-10-17)


Alginate lyase from Bacillus thuringiensis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As it has never reported the cloning of algynate lyase gene in B. thuringiensis we designed the primers using the genome of Bacillus thuringiensis israelensis AM65-52 (GenBank accession number: CP013275.1)

Source

We amplified the alginate lyase gen from the genome of Bacillus thuringiensis 4Q7.

References

(i) FARREL EK, Tipton PA (2012) Functional characterization of AlgL, an alginate lyase from Pseudomona aeruginosa. Biochem. 51:10259-10266. (ii) TIELEN, P, Kuhn H, Rosenau F, Jaeger KE, Flemming HC, Wingender J (2013) Interaction between extracellular lipase LipA and the polysaccharide alginate of Pseudomonas aeruginosa. BMC Microbiol. 159: 221–228.